Internal Solution

Lab Standard

Salt mM
K-Gluconate 120
KCl 20
HEPES 10
NaCl 4
ATP-Mg 4
GTP-Na2 0.3
Phosphocreatine-K2 7

pH 7.3 @ 22oC (Room temperature)

GAP

Salt mM g / ml F.W. Notes
HEPES 10 0.1 M x 0.6371 ml 238.3 Sigma H4034
ATP-Mg 40 0.129 g 507.18 Sigma A9187
GTP-Na2 3 0.01 g 523.18 Sigma G8877
Phosphocreatine-K2 70 0.128 g 287.3 Calbiochem 237911

Final volume = 6.371 (ml)
pH 7.4 @ 0oC (On ice)


HEPES