Internal Solution Notes

Links:

  Johnston's Lab Davie et al
    2006
K-methanesulfate   130
K-Gluconate 120  
KCl 20 7
NaCl 4  
HEPES 10 10
EGTA   0.05
Na2ATP   2
MgATP 4 2
Na2GTP 0.3 0.5
K2-Phosphocreatine 7  
     
K+ 154 137
Na+ 4.6 5
Mg2+ 4 2
Cl- 24 7
     
pH 7.3 7.2
Osmolarity   285

Vargas (1999, p 169-173)

  Δ % PKA activity
KF -80 %
KMeSO4 +139 %
KAsp +116 %
KGluc +113 %
KH2PO4 +180 %
Hepes N.S.
2 mM EGTA/0.5 mM CaCl2 +132 %

Kay (1992, p 91-100)

Lyotrophic effect: F- < SO42- < Cl- < Br- < NO3- < I- < ClO4- < SCN-

Ion Concentration
Na+ 10 ~ 30
K+ 130 ~ 140
Cl- 5 ~ 15
HCO3- 10 ~ 16
A- 109 ~ 155
Mg2+ 0.3 ~ 0.5
Ca2+ 50 ~ 100 nM
ATP 2 ~ 3
Pi 1.7
Phosphocreatine 5
Creatine 6

pH 7.1 ~ 7.3

Cl-

F-


Velumian (1997, p 343-350)


Kaczorowski (2007, p 799-818)